POLARstar Omega
The POLARstar® Omega is the fully-equipped Omega-series microplate reader, offering all the features of a FLUOstar Omega, and in addition fluorescence polarization. Equipped with BMG LABTECH’s ultra-fast UV/vis spectrometer and Simultaneous Dual Emission detection, the POLARstar Omega represents a great combination of performance and flexibility for life science applications.
Product Details
The POLARstar Omega is a versatile, filter-based multi-mode microplate reader with seven detection modes: fluorescence intensity (including FRET), fluorescence polarization, luminescence (including BRET), UV/vis absorbance, time-resolved fluorescence, TR-FRET, and AlphaScreen®/AlphaLISA®.
Features
- Two matched PMTs for Simultaneous Dual Emission
- High-speed absorbance measurements
- Measurements in up to 384-well plates
- Top and bottom reading
- Incubation up to 45 or 65 °C
- Well scanning with up to 900 data points per well
- High-precision injectors with simultaneous reagent injection and detection
- Low volume measurements
Microplate reader technologies
Dedicated high performance luminescence
For the optimization of luminescence assays, BMG LABTECH has created an internal dedicated detection system. This allows for a much higher signal-to-noise ratio than in competing readers and for lower limits of detection. Moreover, specific proprietary luminescence optics increase the signal in various plate formats (96, 384, or 1536), allowing for an uncompromised signal regardless of your plate format.
For the optimization of luminescence assays, BMG LABTECH has created an internal dedicated detection system. This allows for a much higher signal-to-noise ratio than in competing readers and for lower limits of detection. Moreover, specific proprietary luminescence optics increase the signal in various plate formats (96, 384, or 1536), allowing for an uncompromised signal regardless of your plate format.
Well-scanning
For non-homogeneous samples such as for cell-based assays, where the sample distribution is uneven across the well, a single reading in the centre of the well is inadequate. To overcome this problem, all BMG LABTECH readers come with an advanced Well Scanning feature that can take multiple measurements in each well with a resolution of up to 900 points/well. Alternatively, in spiral or orbital averaging mode, the reader takes several measurements for each well on a defined spiral or orbit and calculates an average.
For non-homogeneous samples such as for cell-based assays, where the sample distribution is uneven across the well, a single reading in the centre of the well is inadequate. To overcome this problem, all BMG LABTECH readers come with an advanced Well Scanning feature that can take multiple measurements in each well with a resolution of up to 900 points/well. Alternatively, in spiral or orbital averaging mode, the reader takes several measurements for each well on a defined spiral or orbit and calculates an average.
Simultaneous Dual Emission
Simultaneous Dual Emission (SDE) detection allows the measurement of two emitted wavelengths at the same time. It reduces plate read times and corrects for any signal variation due to differences in well volumes, concentrations, or fluctuations in excitation energy. SDE is the ideal tool for double-emission assays such as FRET, TR-FRET, fluorescence polarization and BRET, since the sample only has to be read once.
Simultaneous Dual Emission (SDE) detection allows the measurement of two emitted wavelengths at the same time. It reduces plate read times and corrects for any signal variation due to differences in well volumes, concentrations, or fluctuations in excitation energy. SDE is the ideal tool for double-emission assays such as FRET, TR-FRET, fluorescence polarization and BRET, since the sample only has to be read once.
Gas Vent
BMG LABTECH offers two forms of gas control on its microplate readers. The Atmospheric Control Unit (ACU) for the CLARIOstar is a unique microprocessor-controlled unit that independently regulates both CO2 and O2 inside the microplate reader. Alternatively, the Gas Vent is a positive pressure gas purge through which the internal atmosphere of the microplate reader can be replaced.
BMG LABTECH offers two forms of gas control on its microplate readers. The Atmospheric Control Unit (ACU) for the CLARIOstar is a unique microprocessor-controlled unit that independently regulates both CO2 and O2 inside the microplate reader. Alternatively, the Gas Vent is a positive pressure gas purge through which the internal atmosphere of the microplate reader can be replaced.
Incubation and shaking
Shaking and incubation allow users to mix the well content and easily run measurements at elevated temperatures. The incubator controls the air temperature in the measurement chamber from room temperature up to 45 °C or 65 °C. BMG LABTECH microplate readers are equipped with orbital, double-orbital or linear shaking with definable intensity, duration and direction of motion. These features are available on all BMG LABTECH plate readers.
Shaking and incubation allow users to mix the well content and easily run measurements at elevated temperatures. The incubator controls the air temperature in the measurement chamber from room temperature up to 45 °C or 65 °C. BMG LABTECH microplate readers are equipped with orbital, double-orbital or linear shaking with definable intensity, duration and direction of motion. These features are available on all BMG LABTECH plate readers.
Path length correction
Absorbance measurements such as DNA quantification or protein determination detected in a microplate reader are frequently compared and/or normalized to results from a 1 cm path length cuvette. All BMG LABTECH microplate readers have the ability to perform a 1 cm path length correction thanks to an internal fitting algorithm. Using this correction, data normalization to 1 cm path is given with only one mouse click.
Absorbance measurements such as DNA quantification or protein determination detected in a microplate reader are frequently compared and/or normalized to results from a 1 cm path length cuvette. All BMG LABTECH microplate readers have the ability to perform a 1 cm path length correction thanks to an internal fitting algorithm. Using this correction, data normalization to 1 cm path is given with only one mouse click.
Reagent injectors
Many of today's most popular assays such as enzyme kinetics, calcium flux, and dual luciferase require the ability to monitor a signal during and after the addition of a reagent.
All BMG LABTECH readers, with the exception of the SPECTROstar Nano , can be equipped with up to two built-in reagent injectors. Injectors enable reagent delivery to any plate format from 6 to 384 wells.
Many of today's most popular assays such as enzyme kinetics, calcium flux, and dual luciferase require the ability to monitor a signal during and after the addition of a reagent.
All BMG LABTECH readers, with the exception of the SPECTROstar Nano , can be equipped with up to two built-in reagent injectors. Injectors enable reagent delivery to any plate format from 6 to 384 wells.
UV/vis spectrometer
BMG LABTECH was the first and is the only microplate reader manufacturer to equip its instruments with a UV/vis spectrometer for absorbance measurements. Similar to a monochromator, but much faster, the spectrometer will capture full-absorbance spectra from 220 to 1000 nm at a resolution of 1 nm in less than one second/well. Alternatively, up to eight discrete wavelengths can be collected at the same time.
BMG LABTECH was the first and is the only microplate reader manufacturer to equip its instruments with a UV/vis spectrometer for absorbance measurements. Similar to a monochromator, but much faster, the spectrometer will capture full-absorbance spectra from 220 to 1000 nm at a resolution of 1 nm in less than one second/well. Alternatively, up to eight discrete wavelengths can be collected at the same time.
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