Monotype HLA

Typing single HLA loci for disease associations and drug sensitivity?

Monotype HLA is a suite of Assay and Software combination products for single-locus HLA typing.

  • The assay leverages the power of the Holotype HLA workflow, providing high throughput and high resolution genotyping to facilitate disease association research, population genetics studies and drug sensitivity testing.

  • The analysis of high resolution HLA data is supported by HLA Twin, the most accurate genotyping software for the processing of NGS data.

Product Highlights

  • Identical protocol for integration with Holotype HLA to maximize flow cell usage
  • Amplify only the loci you need for optimal flow cell usage
  • Pre-sample indexing up to 576 samples per run ***
  • Simple workflow and technician-friendly protocol
  • Automated protocol for pre/post PCR
  • Whole gene amplification
  • Optimized size-selection of fragmented amplicon libraries to help resolve phase and reduce ambiguity
  • Indexed adaptors (avoid bias from 2nd PCR step)
  • Multiple safe stopping points
  • Fully phased whole gene consensus squences
  • Customizable kit configuration
  • Flexible sequencing strategy to support pooling of different libraries
  • Kit splitting and flexibility
  • Elimination of error-prone pipetting steps

All timings are for 192 samples, Monotype HLA v3.0
*     Timings are based on the Biomek 4000 by Beckman Coulter
**   On a MiSeq 300-cycle Micro flow cell
*** Lead time for >192 barcodes varies. Consult your local sales representative for an accurate estimated delivery

What is included in the kit?

  • Primer Component Box for 24 or 96 samples
  • Library Preparation Reagents Component Box for 24 or 96 samples
  • 96 Well Adaptor Plate
  • NGS Excel Workbook
  • HLA Twin – dual algorithm genotyping software

Technical and Equipment Requirements

  • MiniSeq, MiSeq or NextSeq
  • Thermal Cycler
  • Plate fluorometer or qPCR instrument
  • 64-bit computer with a minimum of 16 GB RAM
  • Library Size Selection: Pippin Prep or Magnetic Beads

HLA Disease Associations*

  • Ankylosing spondylitis ( A24)
  • Diabetes, type I (A1, A24)
  • Hematochromatosis (A3)
  • Myasthenia gravis (A4, A24, A30)
  • T-cell adult Leukemia (A26, A28)
  • Multiple Sclerosis (A3)
  • Papilloma virus susept (A11)
  • Spontaneous abortion (A2)
  • Stevens–Johnson syndrome (HLA-B*1502)
  • HIV (B27, B35, B57)
  • Crohn’s disease (B27)
  • Ulcerative colitis (B27)
  • Ankylosing spondylitis (B27)
  • Chronic lymphocytic leukemia(HLA-C*16)
  • Multinodular goiters(Cw1)
  • Anemia (DR5,DR15)
  • Aneurysm (DR16)
  • Arteritis (DR16)
  • Arthritis (DR1, DR4, DR5, DR8, DR12)
  • Cardiomyopathy (DR4)
  • Colitis (DR1)
  • Diabetes (type 1 – DR3, type 2 – DR8)
  • Encephalomyelitis (DR17)
  • Encephalopathy (DR52)
  • Epilepsy (DR5, DR17)
  • Heart disease (DR16)
  • Hepatitis (DR2, DR11)
  • Lupus (DR3, DR4, DR15)
  • Lymphadenopathy (DR5)
  • Myasthenia (DR1, DR3)
  • Myositis (DR17)
  • Nephritis(DR1)
  • Nephropathy(DR4)
  • Pemphigus(DR1, DR4)
  • Psoriasis (DR1)
  • Papillomatosis (DR1)
  • Sarcoidosis (DR17)
  • Sclerosis (DR2, DR3, DR3, DR4, DR12)
  • Schizophrenia (DR1)
  • Susceptibility (DR2, DR3, DR5, DR8, DR10, DR11)
  • Thyroiditis (DR3)
  • Uveitis (DR1)
  • Multiple sclerosis (DRB3*02:02)
  • Graves disease (DRB3*01:01)
  • Childhood ALL (DRB4*01)
  • Lupus (DRB5*01:01)
  • Scleroderoma (DRB5*01:05)
  • Diabetes (type-1 DPA1*01:03)
  • Ankylosing Spondylitis (DPA1*01:03)
  • Rheumatoid arthritis ( DPB1*05:10 )
  • Graves disease ( DPB1*05:01)
  • Multiple sclerosis (DPB1*05:01)
  • Diabetes (DQB1*02:01, DQB1*03:02)
  • Celiac disease ( DQB1*02:01, DQB1*02:02, DQB1 *03:02)
  • Narcolepsy (DQB1*06:02)
  • Multiple sclerosis (DQB1*06:02)
  • Celiac disease(DQA1*05:01)
  • Myasthenia gravis (DQA1*01:01, DQA1*01:02)
  • Podoniosis (DQA1*02:01)
  • Eosinophilia-myalgia syndrome (DQA1*06:01)
  • Achalasia(DQA1*01:03)

*Disclaimer: This list is not complete. There are a lot of new discoveries and the list will be updated regularly.