A revolutionary Holotomographic Microscope to look instantly inside living cells in 3D
Live Cell Imaging with the 3D Cell Explorer: Take your Cell Research to the Next Dimension
NON-INVASIVE 3D CHARACTERIZATION
Live cell imaging in physiological conditions without any bleaching or phototoxicity
LABEL-FREE 4D CONTINUOUS OBSERVATION
Measurement of cell processes from seconds to weeks
MULTIPLEXING
High resolution and high sensitivity characterization of multiple cell organelles based on their refractive index
Non-invasive characterization of live cells in physiological conditions
The 3D Cell Explorer measures the quantitative refractive index of cell organelles in seconds. Hence, it is possible to do non-invasive in vitro imaging of almost any kind of cells with up to 30 μm depth of reconstruction. This allows for biological features to be segmented based on their physical characteristics.
Mouse embryonic stem cells (mESCs) undergoing cell division: 2D slice from RI map in grayscale and 3D reconstructions digitally stained.
Label-free continuous observation of cell processes from seconds to weeks
Study cell life cycle processes of growth, division & death in 3D and 4D. Thanks to a dedicated top-stage incubator you can monitor cell compartments and their kinetics and dynamics in real-time without interfering with their natural physiology.Multiplexing
High resolution and high sensitivity characterization of multiple cell organelles based on their refractive index. Explore and measure different cell compartments with unprecedented detail and resolution, marker-free and preparation-free based on their own physical density.
Mouse fibroblasts and their internal compartments: a. mitochondr ia; b. plasma membrane; c. actin fibers; d. lipid droplets; e. lysosomes; f. nuclear envelope, nucleus & nucleoli.
Explore a new vision
Label-free unstained 3D cells
Long observation time
New space for discoveries
3D Data Sets
Multiplexing, unique organelle segmentation
Quantitative data analysis
Save Experimental Time
No sample preparation
Short setup time
Fast & easy acquisition
Compatible Sample Stage
Top-stage incubator, pipettors, microfluidic devices, …
Features
| Resolution | Δx,y = 200nm; Δz = 400nm |
|---|---|
| Field of View | 85 x 85 x 30 μm |
| Tomography frame rate | 0.5 fps 3D image rate with full self-adjustement |
| Microscope Objective | Air with 60x magnification |
| Illumination Source | Class 1 laser low power (λ=520 nm, sample exposure 0.2 mW/mm2) |
| Accessible Sample Stage | 60 mm of free access to the sample stage for sample manipulation |
| Dimensions | 38 cm x 17 cm x 45 cm |