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Products >> Molecular Biology & Diagnostics >> Reagents & Kits >> Classical PCR Kits
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SACACE develops, manufactures more than 120 complete kits for human, veterinary and food diagnostics. These kits include all the reagents for nucleic acid extraction, amplification (PCR or RT-PCR) of specific region and detection of the amplified products.

High specificity, exquisite sensitivity, very high stability and reproducibility associated with quite competitive prices are characteristic of all these products.

Complete Systems for human, veterinary and food diagnostic, including all the reagents for nucleic acid extraction, reverse transcription, amplification of specific genomic regions (mix in ready to use single dose test tubes) and detection by:

	Agarose gel electrophoresis
	Hybridization-based Enzyme Analysis
	Real Time Amplification

The kit includes RNA/DNA Extraction Kit, Reverse Transcription and Amplification Kit as required by each kit.

PCR kits available for :

1) Virology Kits/Agarose gel based Kits
2) Bacteriology kits/Agarose Gel
3) Fungic & Protozoan Infections kits/Agarose Gel
4) Human genome mutations/ Agarose Gel

1) Virology Kits/Agarose gel based Kits

Code	Kit	Description	N. test
HCV 240/440 IC
V-1-50R V-1-100R	HCV – 240/440 IC	Reverse Transcription and Amplification Kit	55 110
HCV Genotype
V-1-G/50R	HCV – Genotype/1a, 1b, 2, 3	Reverse Transcription and Amplification Kit	55
HGV 340/625 IC
V-2-50R V-2-100R	HGV -340/625 IC	Reverse Transcription and Amplification Kit	55 110
HDV 255/500 IC
V-3-50R V-3-100R	HDV 255/500 IC	Reverse Transcription and Amplification Kit	55 110
HAV 430
V-4-50R	HAV 430	Reverse Transcription and Amplification Kit	55
HBV 470/770 IC
V-5-50R V-5-100R	HBV 470/770 IC	Amplification Kit	55 110
CMV 500/800 IC
V-7-50R V-7-100R	CMV 500/800 IC	Amplification Kit	55 110
HSV 430/720 IC
V-8-50R V-8-100R	HSV 430/720 IC	Amplification Kit	55 110
EBV 290
V-9-50R V-9-100R	EBV 290	Amplification Kit	55 110
HHV-6 380
V-10-50R V-10-100R	HHV-6 380	Amplification Kit	55 110
HPV 6/11
V-11-50F	HPV 6/11	Amplification Kit	55
HPV 16/18
V-12-50R V-12-100R	HPV 16/18	Amplification Kit	55 110
HPV High Risk Screen
V-26 -50F V-26-100F	HPV High Risk Screen (12 types)	Amplification Kit	55 110
HPV High Risk Typing
V-25 -50F	HPV High Risk Typing (detection and genotyping of HPV:16, 18, 31, 33, 35, 39, 45, 52, 56, 58, 59, 66)	Multiplex – Amplification Kit	55
Rotavirus 280
V-15-50R	Rotavirus 280	Reverse Transcription and Amplification Kit	55
Enterovirus 207
V-16-50R	Enterovirus 207	Reverse Transcription and Amplification Kit	55
Norwalk-Like Virus type I/II
V-27-50R	Norwalk-Like Virus type I/II	Reverse Transcription and Amplification Kit	55
Astrovirus 175
V-19-50R	Astrovirus 175	Reverse Transcription and Amplification Kit	55
Flavivirus 215
V-20-50R	Flavivirus 215	Reverse Transcription and Amplification Kit	55
Congo/Crimea 281/504 IC
V-22-50R	Congo/Crimea 281/504 IC	Reverse Transcription and Amplification Kit	55
Adenovirus 455
V-23-50R	Adenovirus 455	Amplification Kit	55
Rosolia 302
V-24-50R	Rosolia 302	Reverse Transcription and Amplification Kit	55
SARS 221/400 IC
V-28-50R	SARS 221/400 IC	Reverse Transcription and Amplification Kit	55
Avian Flu Screening & Typing H5, H7
V-34-50R	Avian Flu Screening & Typing H5, H7	Reverse Transcription and Amplification Kit	55
Influenza Virus A,B
V-38-50R	Influenza virus A,B	Reverse Transcription and Amplification Kit	55
Respiratory syncytial virus (hRSV) 298/550 IC
V-37-50R	Respiratory syncytial virus (hRSV) 298/550 IC	Reverse Transcription and Amplification Kit	55
Xantavirus 475/641 IC
V-39-50R	Xantavirus 475/641 IC	Reverse Transcription and Amplification Kit	55

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2) Bacteriology kits/Agarose Gel

Code	Kit	Description	N. test
Chlamydia trachomatis 330/700 IC
B-1-50R B-1-100R	Chlamydia trachomatis 330/700 IC	Amplification Kit	55 110
Ureaplasma urealyticum 450/750 IC
B-2-50R B-2-100R	Ureaplasma urealyticum 450/750 IC	Amplification Kit	55 110
Mycoplasma hominis 330/550 IC
B-3-50R B-3-100R	Mycoplasma hominis 330/550 IC	Amplification Kit	55 110
Mycoplasma genitalium 280/550 IC
B-4-50R B-4-100R	Mycoplasma genitalium 280/550 IC	Amplification Kit	55 110
Neisseria gonorrhoeae 370/660 IC
B-5-50R B-5-100R	Neisseria gonorrhoeae 370/660 IC	Amplification Kit	40+15 80+30
Trichomonas vaginalis 240/520 IC
B-6-50R B-6-100R	Trichomonas vaginalis 240/520 IC	Amplification Kit	55 110
Gardnerella vaginalis 355/640 IC
B-7-50R B-7-100R	Gardnerella vaginalis 355/640 IC	Amplification Kit	55 110
Corinebact. Diphteriae 380
B-8-50R	Corinebacterium diphteriae 380	Amplification Kit	55
Helicobacter pylori 520
B-9-50R	Helicobacter pylori 520	Amplification Kit	55
Brucella species 460/770 IC
B-10-50R	Brucella species 460/770 IC	Amplification Kit	55
Salmonella species 225/670 IC
B-11-50R	Salmonella sp. 225/670 IC	Amplification Kit	55
Shigella species 400/800 IC
B-12-50R	Shigella species 400/800 IC	Amplification Kit	55
Campilobacter species 520
B-35-50R	Campilobacter species 520	Amplification Kit	55
Lysteria monocytogenes 280
B-14-50R	Lysteria monocytogenes 280	Amplification Kit	55
MTB complex 390/750 IC
B-15-50R B-15-100R	MTB complex 390/750 IC	Amplification Kit	55 110
Mycoplasma species 500
B-16-50R	Mycoplasma species 500	Amplification Kit	55
Chlamydiaceae complex 300
B-17-50R	Chlamydiaceae complex 300	Amplification Kit	55
Streptococcus species 340
B-18-50R	Streptococcus species 340	Amplification Kit	55
Ureaplasma Parvo/T-960
B-19-50R	Ureaplasma – (Parvo/T-960)	Amplification Kit	55
Treponema pallidum 273/668 IC
B-20-50R B-20-100R	Treponema pallidum 273/668 IC	Amplification Kit	55 110
Clostridium difficile 420
B-23-50R	Clostridium difficile 420	Amplification Kit	55
NHS Meningitis
B-25-50R	NHS Meningitis (detection and identification of Neisseria meningitidis, Haemophilus influenzae, Streptococcus pneumoniae)	Amplification Kit	55
Neisseria meningitidis A, B, C types
B-26-50R	Neisseria meningitidis A, B, C types (detection and identification of Neisseria meningitidis type A, B, C)	Amplification Kit	55
Borrelia burgdorferi sensu lato
B-37-50R	Borrelia burgdorferi sensu lato (B.burgdorferi sensu stricto, B. afzelii, B. garinii)	Amplification Kit	55
Mycoplasma pneumoniae 325/565 IC
B-40-50R	Mycoplasma pneumoniae 325/565 IC	Amplification Kit	55
Mycobacterium paratuberculosis 209/700 IC
B-38-50R	Mycobacterium paratuberculosis 209/700 IC	Amplification Kit	55
Yersinia pseudotuberculosis 527
B-39-50R	Yersinia pseudotuberculosis 527	Amplification Kit	55

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3) Fungic & Protozoan Infections kits/Agarose Gel

Code	Kit	Description	N. test
Candida albicans 500/730 IC
F-1-50R F-1-100R	Candida albicans 500/730 IC	Amplification Kit	55 110
Toxoplasma gondii 325
P-1-50R P-1-100R	Toxoplasma gondii 325	Amplification Kit	55 110

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4) Human genome mutations/ Agarose Gel

Code	Kit	Description	N. test
Amplikit MTHFR C677T
0503	Methylenetetrahydrofolate reductase (MTHFR), 677 C/T Applications: Atherosclerosis, arterial and venous thrombosis, obstetrics complications	Amplification of human genomic DNA region surrounding the position 677 of the methylenetetrahydrofolate reductase gene, and subsequent restriction fragments length polymorphism analysis	50
Amplikit PRT
0502	Coagulation factor II (prothrombin), 20210 G/A Applications: Venous and arterial thrombosis, obstetrics complications	Amplification of human genomic DNA region surrounding the position 20210 of the factor II gene, and subsequent restriction fragments length polymorphism analysis	50
Amplikit FVL
0501	Coagulation factor V, 1691 G/A (FV Leiden) Applications: Venous thrombosis, obstetrics complications, arterial thrombosis at young age	Amplification of human genomic DNA region surrounding the position 1691 of the factor V gene, and subsequent restriction fragments length polymorphism analysis	50
AmpliKit Gilbert's syndrome (A(TA)6TAA/A (TA)7TAA)
010723	UGT1A1 A(TA)6TAA/A (TA)7TAA	Amplification with sequence-specific primers	50
AmpliKit Cystic Fibrosis Asn1303Lys
010717	CFTR Asn1303Lys	Amplification with sequence-specific primers	50
AmpliKit Cystic Fibrosis Trp1282Ter
010716	CFTR Trp1282Ter	Amplification with sequence-specific primers	50
AmpliKit Cystic Fibrosis Gly551Asp
010715	CFTR Gly551Asp	Amplification with sequence-specific primers	50
AmpliKit Cystic Fibrosis Gly542Ter
010714	CFTR Gly542Ter	Amplification with sequence-specific primers	50
AmpliKit Cystic Fibrosis Phe508Del
010713	CFTR Phe508Del	Amplification with sequence-specific primers	50
AmpliKit ATPase, Cu++ transporting, beta polypeptide Gly1267Arg (Wilson disease)
010722	ATP7B Gly1267Arg	Amplification with sequence-specific primers	50
AmpliKit ATPase, Cu++ transporting, beta polypeptide del C3402 (Wilson disease)
010721	ATP7B del C3402	Amplification with sequence-specific primers	50
AmpliKit ATPase, Cu++ transporting, beta polypeptide His1069Gln (Wilson disease)
010712	ATP7B His1069Gln	Amplification with sequence-specific primers	50
AmpliKit Aldehyde dehydrogenase ALDH2
010709	*ALDH2 Glu487Lys (ALDH22)**	Amplification with sequence-specific primers	50
AmpliKit Alcohol dehydrogenase ADH1B
010708	*ADH1B Arg47His (ADH21/ADH22)*	Amplification with sequence-specific primers	50
AmpliKit FVII Arg353Gln
010704	FVII Arg353Gln	Amplification with sequence-specific primers	50
Amplikit ENOS
0601	Endothelial Nitric Oxide Synthase eNOS 786 T/C Applications: Hypertension, atherosclerosis, venous and arterial thrombosis, obstetrics complications	PCR mediated amplification of human genomic DNA region surrounding the position “–786” of the nitric oxide endothelial synthetase (eNOS) gene, and subsequent restriction fragments length polymorphism analysis (PCR-RFLP)	50
Amplikit MTHFD1
0602	Methylenetetrahydrofolate dehydrogenase (MTHFD1), 1958 G/A Applications: Therapeutic response to antifolate chemotherapeutics in acute lymphoblastic leukemia, risk of pregnancy loss, risk of neural tube defect, risk of abruptio placentae, atherosclerosis, venous and arterial thrombosis	PCR mediated amplification of human genomic DNA region surrounding the position 1958 of the MTHFD1 gene, and subsequent restriction fragments length polymorphism analysis (PCR-RFLP)	50
Amplikit ESTR
0603	Estrogen receptor (ER-α), IVS-1- 397T/C Applications: Myocardial infarction, intracerebral hemorrhage, osteoporosis, breast cancer, neurodegenerative diseases	PCR mediated amplification of human genomic DNA region surrounding the position –397 of the ER-α gene intron 1 (IVS-1), and subsequent restriction fragments length polymorphism analysis (PCR-RFLP)	50
Amplikit VDRF
0604	Vitamine D receptor D (VDR), start codon F/f Applications: Osteoporosis, tuberculosis, prostate cancer, breast cancer, urolithiasis	PCR mediated amplification of human genomic DNA region surrounding start codon of the vitamin D receptor (VDR) gene, and subsequent restriction fragments length polymorphism analysis (PCR-RFLP)	50
Amplikit GPIA
0505	Glycoprotein Ia (GpIa), 807 C/T Applications: Atherosclerosis, arterial and venous thrombosis, obstetrics complications	Amplification of human genomic DNA region surrounding the position 807 of the glycoprotein Ia gene, and subsequent restriction fragments length polymorphism analysis	50
Amplikit HPA-1
0506	Glycoprotein IIIa (GpIIIa), 1565 T/C (PIA1/A2) Applications: Arterial and venous thrombosis, obstetrics complications, platelet transfusions	Amplification of human genomic DNA region surrounding the position 1565 of the glycoprotein IIIa gene, and subsequent restriction fragments length polymorphism analysis	50
Amplikit HPA-2
0507	Glycoprotein Ibα (GpIbα), 434 C/T (Thr145Met) Applications: Arterial and venous thrombosis, obstetrics complications, platelet transfusions	Amplification of human genomic DNA region surrounding the position 434 of the glycoprotein Ibα gene, and subsequent restriction fragments length polymorphism analysis	50
Amplikit HPA-3
0509	Glycoprotein IIb (GpIIb), 843 IIe/Ser Applications: platelet transfusions	Amplification of human genomic DNA region surrounding the position 843 of the glycoprotein IIb, and subsequent restriction fragments length polymorphism analysis	50
Amplikit PAI-1
0511	Plasminogen activator inhibitor type I, -675 4G/5G Applications: Arterial and venous thrombosis, obstetrics complications	Amplification of human genomic DNA region surrounding the position 675 and subsequent restriction fragments length polymorphism analysis	50