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Real Time DNA Amplification
System
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REAL
TIME PCR WITH SMART CYCLER II (Cepheid, USA)
The
Smart Cycler II is built with sixteen modular I-CORE
(Intelligent Cooling/Heating optical Reaction) modules.
Each independent module must maintain tight temperature
specifications, established with National Institute of
Standards Technology (NIST)- traceable standards.
Real time detection
Combine amplification and detection in one
step. Fluorescence is measured in real time
as PCR products are generated.
Flexible
Easy assay optimization &expedited with the
ability to run a different protocol in each
of the sixteen sites simultaneously. The
ability for multiple operations to use the
system concurrently gives new meaning to the
term multi-tasking.
Fast
Results are available in as little as 20
minutes due to very rapid, accurate heating
and cooling cycles.
Four- channel optics
Develop multiplex assays with the ability to
detect fluorescent signal in four distinct
channels
Expandable
Link up to six processing blocks for cost-
effective system expansion
Display options
Protocol definitions, data analysis
including primary curve and second
derivative analysis, fluorescence &
temperature data graphs
Data management (
with advanced Smart Cycler software version
2.0)
Manage runs with controlled ramp rates
Automatically advance to next stage after
crossing threshold Define default data
analysis and data access Compare runs or
import a standard curve Save graphs as JPEG
files Automatically export and /or back up
data
Optical blocks
High intensity LEDs, silicon photo
detectors, and multiple filters allow
simultaneous excitation and detection for
four different spectral bands.
Four optical channels
Smart Cycler II allows measurement of
fluorescence in four channels at the same
time. High intensity LEDs and optical
filters are optimized for spectral
separation.
Instrument calibration allows the use of :
Channel 1 FAM or SYBR green
Channel 2 Cy3 or TET
Channel 3 Texas red (TxR)
Channel 4 Cy5
Multiplex capability
Allows detection of several targets within a
single sample
Use of multiple fluorescent dyes permits
detection of signal in up to four channels
at one time on the Smart Cycler system.
Various detection chemistries
Taqman and Molecular Beacons probes,
Amplifluor and Scorpion primers and
intercalating dyes such as SYBR green.
Sensitivity
Sensitivity on the Smart Cycler is affected
by many factors, including the quality of
template preparation, primer and probe
design and optimization of PCR conditions.
Orthopox assay illustrates- Quantitative low
copy detection
