Products >> Drug Discovery & Screening >>Assay Kits >> GPCR Targets
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Assay any GPCR using a single technology

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Gs, Gi and Gq Functional Assays

GPCRs carry information within cells via two major signaling pathways: regulation of cAMP levels and increases in intracellular Ca2+ triggered by inositol (1,4,5) tri-phosphate (IP3). These signaling pathways are activated by the specific G protein associated with the receptor. Gs and Gi coupled receptors result in variations of cAMP while Gq coupled GPCRs activate phospholipase C (PLC) and trigger the inositol phosphate (IP) cascade. Cisbio has developed a full line of quality, ready-to-use kits capable of monitoring activation of Gs, Gi and Gq coupled receptors.

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Features :

	Single technology for all GPCR assays
	Simple and rapid assay protocols
	Stable EC50 even after several day incubation
	Miniaturization down to < 10µl
	Low false positive, false negative results

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Applications :

	Gs, Gi and Gq coupled receptor activation
	Agonist / antagonist screening
	Inverse agonist testing for constitutively active receptors
	Phosphodiesterase (PDE) monitoring
	Adenylate cyclase activity assessment

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