NFκB Reporter A549, HeLa, 293, NIH3T3, and C2C12 Stable Cell Lines

	Monitor biochemical changes in NFκB pathway
	Screen compounds that inhibit or activate any pathway components
	No hassle or reporter construct transfection

Panomics has established NFκB reporter stable cells in a number of popular cell lines suitable for specific applications in the study of NFκB. They include human embryonic kidney cells 293, human cervical epithelial cells HeLa, mouse fibroblast cells NIH3T3, human lung carcinoma cells A549 and mouse myoblast C2C12 cells. All these cell lines have been used extensively for the study of NFκB signaling due to their robust response. The 293 and HeLa are highly transfectable human cell lines whereas NIH3T3 cells are murine cells easy to maintain and transfect. In addition, A549 cells and C2C12 cells are useful cell models for studying the role of NFκB in lung carcinogenesis and muscle differentiation, respectively.

NFκB background
Nuclear Factor kappa B (NFκB) is a member of the rel family of transcription factors. NFκB plays a key role in the regulation of inflammatory response, apoptosis or tumorigenesis.

NFκB is activated by a wide variety of different stimuli such as pro inflammatory cytokines, oxidant free radicals, inhaled particles, ultraviolet radiation and bacterial or viral products. In the inactive state, NFκB forms a cytoplasmic complex with Inhibitory kappa B Protein (IκB). Upon stimulation, several types of kinases belonging to the mitogen-activated protein kinase (MAPK) family activate NFκB as follows: first, IκB is phosphorylated, dissociated and degraded. The NFκB protein then translocates to the nucleus where it binds to its specific DNA motifs and initiates transcription of genes. NFκB regulates the expression of many genes which play a role in immunity and inflammation e.g. IL2, TNF, adhesion molecules and acute phase proteins.

The NFκB family consists of a number of structurally related members (p50, RelA(p65), RelA, RelB, RelC). These are dimeric (homo and hetero) complexes containing various combinations of these proteins. The members of the NFκB protein group, presently best characterized are the p50/p65(RelA) heterodimer and the p50/p50 homodimer complexes.

With Panomics’ NFκB Reporter Stable Cell Line you can accurately monitor any changes occurring along the NFκB pathway. The stable cell lines are derived from human A549, HeLa, 293, NIH3T3, and C2C12 cells with chromosomal integration of a luciferase reporter construct regulated by 6 copies of the NFκB response element. This clonal cell line is obtained by cotransfection of pNFκB-luc and pTK-hyg followed by hygromycin selection at 200 μg/ml.