Sa Cycler - 96

96 Well, Real Time PCR system, Sacace, Italy

  • 4 or 5 channels multiplexing for discrimination of up to five targets in a single reaction well
  • Strong flexibility thanks to the 96-well format suited for standard PCR microplates, test tubes and strips
  • Possibility to use tubes with different heights with regulation of the matrix block
  • Optimal signal/noise ratio and absence of crosstalk ensured by the unique design of the optical track including a separate light source for each channel and a matrix CCD camera
  • Light emitting diodes (LED) as a light source with a lifetime of about 100,000 hours that does not require maintenance or constant
  • Wide dynamic range of detection using multiple exposure method, which leads the optimization of signal registration conditions to a whole new level, greatly simplifying or even eliminating the need for fluorescence settings
  • Main applications are Real-Time quantitation, single nucleotide polymorphisms (SNPs) genotyping, melting curve and gene expression analysis
  • Instrument comes with the most used Sacace protocols already installed in the software minimizing possibility of error during programming
  • Melting & SNP analysis.

Optical Scheme

Specifications - Quantitative and Qualitative Analysis

Qualitative analysis allows to determine presence or absence of target DNA in a sample.

Quantitative analysis, with the use of calibration standards, allows to determinate the quantity of target DNA in the sample.

It is possible to use calibration standards from other run files.

Relative concentration analysis allows to evaluate the quantity of the target DNA fragment in the analyzed samples relative to the “reference” test tube.

By interpolating the Ct value of the samples on the standard curve it is possible to measure the actual quantity of target DNA in the samples.

Melting curves are applied to analysis for determination of polymorphisms of single nucleotides.

Real time PCR analysis of genetic polymorphisms like SNP with the use of two different fluorophores in one test tube.

To learn more about the SaCycler Real Time PCR system, please contact our application support team at or Toll Free number 18002670607

1800 267 0607

+91-22-2528 1012

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